Carlos Marques

Lipid oxidation induces structural changes in biomimetic membranes

Carlos Marques — Wed, 01 Jan 2014 12:21:40 +0000

Soft Matter, 10, 4241-4247 (2014) G. Weber, T. Charitat, M. S. Baptista, A. F. Uchoa, C. Pavani, H. C. Junqueira, Y. Guo, V. A. Baulin, R. Itri, C. M. Marques, A. P. Schroder Oxidation can intimately influence and structurally compromise the levels of biological self-assembly embodied by intracellular and plasma membranes. Lipid peroxidation, a natural metabolic outcome of life with oxygen under light, is also a salient oxidation reaction in photomedecine treatments. However, the effect of peroxidation on the fate of lipid membranes remains elusive. Here we use a new photosensitizer that anchors and disperses in the membrane to achieve spatial

(Read more...)

ESR7-S: Optical microscopy and microrheology of giant unilamellar vesicles interacting with nano-objects

Carlos Marques — Thu, 05 Dec 2013 15:09:11 +0000

Experimental investigation of unilamellar vesicles and their dynamics Description Experimental project on the morphological and mechanical changes in lipid vesicles caused by exposure to the nano-objects, by means of epi- and confocal fluorescence studies. Emphasis will be put on understanding the influence of lipid composition, oxidation and investigating the membrane structure changes using ratiometric fluorescent probes. Starting date December 2014 Methods and techniques Related research topics

(Read more...)

ESR8-S: Kinetics of nanoscale structural changes occurring in small unilamellar vesicles

Carlos Marques — Thu, 05 Dec 2013 15:07:02 +0000

Experimental investigation of unilamellar vesicles using microscopy techniques Contact Monika Kluzek Description Experimental project on small unilamellar lipid vesicles (SUV) interacting with nanoparticles, with special emphasis on the use of transmission electron microscopy (cryo-TEM). This project will consist in elaborating, characterizing and studying in detail decorated liposomes, using state of the art techniques such as EM tomography and time controlled freezing. He/she will become the referent for the electron microscopy platform. Project start July 2014 Related research topics

(Read more...)