Laser Scanning Confocal Microscopy (LSCM)

Laser Scanning Confocal Microscopy (LSCM) is a fluorescence microscopy technique widely used in the biological and medical field for imaging thin optical sections in living and fixed specimens . Particularly in biophysics and biology it has become an essential tool not only to visualize lipid model membranes, but also to perform spectral imaging and diffusion rate of fluorescent probes using fluorescence recovery after bleaching (FRAP). The key principle behind confocal approach is to spatially filter the out-of focus light in specimens whose thickness exceeds the plane of focus to improve contrast and definition by discarding the background fluorescent emission from … Continue reading Laser Scanning Confocal Microscopy (LSCM)