Posts by Monika Kluzek published at ITN SNAL - Marie Curie Initial Training Network

Kinetic evolution of DOPC lipid bilayers exposed to α-cyclodextrins

Monika Kluzek — Fri, 06 Jul 2018 12:19:09 +0000

Soft Matter, , (2018) Monika Kluzek, Marc Schmutz, Carlos M. Marques and Fabrice Thalmann Cyclodextrins are cyclic oligosaccharides capable of forming inclusion complexes with a variety of molecules, and as such have been recognized as a pharmaceutical and biotechnological asset. Cyclodextrins are known to interact with the components of cell membranes, and this correlates with a significant degree of cytotoxicity. In this work, we report on the mechanism of degradation of a model dioleoyl-phosphatidylcholine (DOPC) bilayer exposed to a solution with increasing con- centrations of α-cyclodextrins. By combining optical fluorescence microscopy and quartz-crystal microbalance experiments, we study the evolution of

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Influence of a pH-sensitive polymer on the structure of monoolein cubosomes

Monika Kluzek — Fri, 05 Jan 2018 11:53:33 +0000

Soft Matter, 13, 7571-7577 (2017) Monika Kluzek, Arwen I. I. Tyler, Shiqi Wang, Rongjun Chen, Carlos M. Marques, Fabrice Thalmann, John M. Seddon, Marc Schmutz Cubosomes consist in submicron size particles of lipid bicontinuous cubic phases stabilized by surfactant polymers. They provide an appealing road towards the practical use of lipid cubic phases for pharmaceutical and cosmetic applications, and efforts are currently being made to control the encapsulation and release properties of these colloidal objects. We overcome in this work the lack of sensitivity of monoolein cubosomes to pH conditions by using a pH sensitive polymer designed to strongly interact

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Fundamentals and applications of lipid membrane biophysics

Monika Kluzek — Thu, 15 Oct 2015 18:02:04 +0000

Horsley Park, 22nd-24th September 2015 Researchers from the ITN-SNAL network have met again to discuss the frontiers in the biophysics and biochemistry of lipid membranes. Throughout the two-day meeting (22.09.2015 – 24.09.2015) held in Horsley Park, UK, young researchers had a unique opportunity for active interactions with experts in the field. By showing their latest results and discussing conundrums that have arisen along the way, ITN PhD students gained fresh yet critical insights into their projects, along with suggestions to broaden their work towards more realistic applications. The meeting provided an opportunity for interactions both between researchers within the ITN-SNAL

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Strasbourg: Principles of Imaging for Membrane systems

Monika Kluzek — Sat, 29 Aug 2015 19:36:14 +0000

Dates: 30 November – 3 December 2015 Location: Institut Charles Sadron (23, Rue de Loess, 67034, Strasbourg, France) Core training courses will cover such topics as methodology, models, and statistical estimation and hypothesis tests. Essential background and computational models for modelling lipid bilayers and their interactions with nano-objects will also be provided. Given the large differences in background between experimental and theoretical/computational ESRs, training courses will aim to highlight these two areas at different levels. Principles of imaging for membrane systems Courses will be given by senior academics in Strasbourg. A three days introduction to theoretical and experimental aspects of optical and

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ESR8-S: Kinetics of nanoscale structural changes occurring in small unilamellar vesicles

Monika Kluzek — Thu, 05 Dec 2013 15:07:02 +0000

Experimental investigation of unilamellar vesicles using microscopy techniques Contact Monika Kluzek Description Experimental project on small unilamellar lipid vesicles (SUV) interacting with nanoparticles, with special emphasis on the use of transmission electron microscopy (cryo-TEM). This project will consist in elaborating, characterizing and studying in detail decorated liposomes, using state of the art techniques such as EM tomography and time controlled freezing. He/she will become the referent for the electron microscopy platform. Project start July 2014 Related research topics

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