Posts by Mattia Morandi published at ITN SNAL - Marie Curie Initial Training Network

Strasbourg: Principles of Imaging for Membrane systems

Mattia Morandi — Sat, 29 Aug 2015 19:36:14 +0000

Dates: 30 November – 3 December 2015 Location: Institut Charles Sadron (23, Rue de Loess, 67034, Strasbourg, France) Core training courses will cover such topics as methodology, models, and statistical estimation and hypothesis tests. Essential background and computational models for modelling lipid bilayers and their interactions with nano-objects will also be provided. Given the large differences in background between experimental and theoretical/computational ESRs, training courses will aim to highlight these two areas at different levels. Principles of imaging for membrane systems Courses will be given by senior academics in Strasbourg. A three days introduction to theoretical and experimental aspects of optical and

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Laser Scanning Confocal Microscopy (LSCM)

Mattia Morandi — Wed, 29 Oct 2014 12:51:11 +0000

Laser Scanning Confocal Microscopy (LSCM) is a fluorescence microscopy technique widely used in the biological and medical field for imaging thin optical sections in living and fixed specimens . Particularly in biophysics and biology it has become an essential tool not only to visualize lipid model membranes, but also to perform spectral imaging and diffusion rate of fluorescent probes using fluorescence recovery after bleaching (FRAP). The key principle behind confocal approach is to spatially filter the out-of focus light in specimens whose thickness exceeds the plane of focus to improve contrast and definition by discarding the background fluorescent emission from

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ESR7-S: Optical microscopy and microrheology of giant unilamellar vesicles interacting with nano-objects

Mattia Morandi — Thu, 05 Dec 2013 15:09:11 +0000

Experimental investigation of unilamellar vesicles and their dynamics Description Experimental project on the morphological and mechanical changes in lipid vesicles caused by exposure to the nano-objects, by means of epi- and confocal fluorescence studies. Emphasis will be put on understanding the influence of lipid composition, oxidation and investigating the membrane structure changes using ratiometric fluorescent probes. Starting date December 2014 Methods and techniques Related research topics

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