Posts by Martin Stefanic published at ITN SNAL - Marie Curie Initial Training Network https://itn-snal.net/author/mstefanic/ Smart Nano-objects for Alteration of Lipid bilayers Mon, 26 Jun 2017 11:51:52 +0000 en-US hourly 1 https://itn-snal.net/wp-content/uploads/sites/9/2015/09/cropped-ITN-SNAL-69x69.jpg Posts by Martin Stefanic published at ITN SNAL - Marie Curie Initial Training Network https://itn-snal.net/author/mstefanic/ 32 32 Apatite nanoparticles strongly improve red blood cell cryopreservation by mediating trehalose delivery via enhanced membrane permeation https://itn-snal.net/2017/06/apatite-nanoparticles-strongly-improve-red-blood-cell-cryopreservation-mediating-trehalose-delivery-via-enhanced-membrane-permeation/ https://itn-snal.net/2017/06/apatite-nanoparticles-strongly-improve-red-blood-cell-cryopreservation-mediating-trehalose-delivery-via-enhanced-membrane-permeation/#respond Mon, 26 Jun 2017 11:51:52 +0000 https://itn-snal.net/?p=2432 Biomaterials, 140, 138-149 (2017). Martin Stefanic, Kevin Ward, Harvey Tawfik, Ralf Seemann, Vladimir Baulin, Yachong Guo, Jean-Baptiste Fleury, Christophe Drouet Cryopreservation of red blood cells (RBC) is an important method for maintaining an inventory of rare RBC units and managing special transfusion circumstances. Currently, in a clinical setting, glycerol is used as cryoprotectant against freezing damage. After thawing and before transfusion, glycerol must however be removed to avoid intravascular hemolysis, via a complex and time-consuming deglycerolization process which requires specialized equipment. Improved cryopreservation methods using non-toxic agents are required to increase biocompatibility and decrease processing time. Biocompatible cryoprotectants (e.g. trehalose)

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]]> https://itn-snal.net/2017/06/apatite-nanoparticles-strongly-improve-red-blood-cell-cryopreservation-mediating-trehalose-delivery-via-enhanced-membrane-permeation/feed/ 0 ER2-B: Investigation of membrane permeating materials in biostabilisation https://itn-snal.net/2013/12/er2-b-investigation-membrane-permeating-materials-biostabilisation/ https://itn-snal.net/2013/12/er2-b-investigation-membrane-permeating-materials-biostabilisation/#respond Thu, 05 Dec 2013 15:53:59 +0000 http://itn-snal.net/?p=284 WinchesterExperimental methods and characterisation techniques in cell preservation and biostabilisation Objectives Evaluate how size, shape and physical nature (net charge, pH responsiveness and nanoparticle and CNT characteristics) affect their interaction with membranes. These studies aim to link transfection efficiency from different therapeutic models (size, charge and proteins versus DNA) with the structural design of the therapeutic nanoparticles, and to shed more light on the toxicity of such substances in general. Tasks and methodology Testing new pH-responsive synthetic polymers (from Cambridge) to deliver (chemo-)biotherapeutics and stabilisers to the cell cytoplasm by disrupting endosomal membranes, which are used for cell preservation technology.

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