Experimental methods and characterisation techniques in cell preservation and biostabilisation
Evaluate how size, shape and physical nature (net charge, pH responsiveness and nanoparticle and CNT characteristics) affect their interaction with membranes. These studies aim to link transfection efficiency from different therapeutic models (size, charge and proteins versus DNA) with the structural design of the therapeutic nanoparticles, and to shed more light on the toxicity of such substances in general.
Tasks and methodology
Testing new pH-responsive synthetic polymers (from Cambridge) to deliver (chemo-)biotherapeutics and stabilisers to the cell cytoplasm by disrupting endosomal membranes, which are used for cell preservation technology.