Posts by André Dias published at ITN SNAL - Marie Curie Initial Training Network

High-Throughput 3D Visualization of Nanoparticles attached to the Surface of Red Blood Cells

André Dias — Wed, 26 Dec 2018 14:21:44 +0000

Nanoscale, December (2018) André Dias, Marco Werner, Kevin Ward, Jean-Baptiste Fleury, Vladimir A. Baulin Blood circulation is the main distribution route for systemic delivery and the possibility to manipulate red blood cells by attaching nanoparticles to their surface provides a great opportunity for cargo delivery into tissues. Nanocarriers attached to red blood cells can be delivered to specific organs by orders of magnitude faster than if diluted in the blood. Another ad-vantage is a shielding from recognition of the immune system, thereby increasing the efficiency of delivery. We present a high-throughput microfluidic method that can monitor the shape of drifting

(Read more...)

The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells

André Dias — Sun, 31 Dec 2017 13:53:54 +0000

Materials, 11(1) 60 (2018) Anna Orlowska, Pallale Tharushi Perera, Mohammad Al Kobaisi, Andre Dias, Huu Khuong Duy Nguyen, Shahram Ghanaati, Vladimir A. Baulin, Russell J. Crawford, Elena P. Ivanova Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings

(Read more...)

Sugar-based collagen membrane cross-linking increases barrier capacity of membranes

André Dias — Thu, 14 Dec 2017 16:15:51 +0000

Clin. Oral Invest., (2017) Po-ju Chia-LaiAnna OrlowskaSarah Al-MaawiAndre DiasYunxin ZhangXuejiu WangNiklas ZenderRobert SaderC. James KirkpatrickShahram Ghanaati This study examines the permeability and barrier capacity of a sugar cross-linked resorbable collagen membrane ex vivo and in vivo. In an ex vivo study, injectable platelet-rich fibrin (i-PRF), a peripheral blood-derived human leukocyte-and-platelet-rich plasma was used to analyze membrane permeability. in vivo subcutaneous implantation in Wistar rats (n = 4 per time point and group) was used to investigate the barrier capacity of the membrane. The induced in vivo cellular reaction was evaluated at 3, 15, and 30 days and compared to sham OP (control)

(Read more...)

Reduction of the relative centrifugal force influences cell number and growth factor release within injectable PRF-based matrices

André Dias — Thu, 02 Nov 2017 16:10:22 +0000

J Mater Sci: Mater Med, 28, 188 (2017) Simon Wend, Alica Kubesch, Anna Orlowska, Sarah Al-Maawi, Niklas Zender, Andre Dias, Richard J. Miron, Robert Sader, Patrick Booms, C. James Kirkpatrick, Joseph Choukroun, Shahram Ghanaati Platelet rich fibrin (PRF) is a blood concentrate system obtained by centrifugation of peripheral blood. First PRF matrices exhibited solid fibrin scaffold, more recently liquid PRF-based matrix was developed by reducing the relative centrifugation force and time. The aim of this study was to systematically evaluate the influence of RCF (relative centrifugal force) on cell types and growth factor release within injectable PRF- in the range of

(Read more...)

ESR11-F: Delivery of molecular payloads through primary human cell membrane

André Dias — Thu, 05 Dec 2013 15:44:08 +0000

In-vitro experiments on living cells, toxicology studies Contact Andre Dias Objectives The goal is to compare and validate the synthetic lipid models to biologically relevant cell types and to investigate how different nano-objects interact with representative lipid single and bilayers compared to human cells with and without therapeutic incorporation. Tasks and methodology Cellular uptake of fluorescence-labelled nano-complexes will be checked by Delta Vision High Resolution microscopy and with an accompanied immunofluorescence counter-staining for intracellular organelles to identify distinct cellular endocytotic routes such as clathrin- or caveolae-dependent mechanisms or intracellular dispositions of the nanoparticles. Nanoparticles will be applied to the upper

(Read more...)