Elena Ivanova

High Aspect Ratio Nanostructures Kill Bacteria via Storage and Release of Mechanical Energy

Elena Ivanova — Mon, 25 Jun 2018 13:10:56 +0000

ACS Nano, 114(9), 2165–2173 (2018) Denver P. Linklater, Michael De Volder, Vladimir A Baulin, Marco Werner, Sarah Jessl, Mehdi Golozar, Laura Maggini, Sergey Rubanov, Eric Hanssen, Saulius Juodkazis, Elena P. Ivanova The properties of lipid bilayers in sucrose solutions have been intensely scrutinized over recent decades because of the importance of sugars in the field of biopreservation. However, a consensus has not yet been formed on the mechanisms of sugar-lipid interaction. Here, we present a study on the effect of sucrose on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers that combines calorimetry, spectral fluorimetry, and optical microscopy. Intriguingly, our results show a significant decrease in

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Mechano-bactericidal mechanism of graphene nanomaterials

Elena Ivanova — Sat, 28 Apr 2018 12:48:05 +0000

Interface Focus, 8, 20170060, (2018) Denver P. Linklater, Vladimir A. Baulin, Saulius Juodkazis and Elena P. Ivanova Growing interest in the bactericidal effect of graphene and graphene-derived nanomaterials has led to the investigation and effective publication of the bactericidal effects of the substratum, many of which present highly conflicting material. The nature of bacterial cell death on graphene bio-interfaces, therefore, remains poorly understood. Here, we review recent findings on the bactericidal effect of graphene and graphene-derived nanomaterials, and proposed mechanisms of cell inactivation, due to mechanical contact with graphene materials, including lipid extraction, physical damage to membranes and pore formation. DOI: 10.1098/rsfs.2017.0060

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Study of melanin localization in the mature male Calopteryx haemorrhoidalis damselfly wings

Elena Ivanova — Sat, 28 Apr 2018 12:38:09 +0000

J. Synchrotron Rad. 25, 874-877 (2018) Vi Khanh Truong, Jitraporn Vongsvivut, Nipuni Mahanamanam Geeganagamage, Mark J. Tobin, Pere Luque, Vladimir A. Baulin, Marco Werner, Shane Maclaughlin, Russell J. Crawford and Elena P. Ivanova Damselflies Calopteryx haemorrhoidalis exhibiting black wings are found in the western Mediterranean, Algeria, France, Italy, Spain and Monaco. Wing pigmentation is caused by the presence of melanin, which is involved in physiological processes including defence reactions, wound healing and sclerotization of the insect. Despite the important physiological roles of melanin, the presence and colour variation among males and females of the C. haemorrhoidalis species and the localization of the pigment within the wing membrane remain poorly

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Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces

Elena Ivanova — Sat, 28 Apr 2018 12:27:39 +0000

Materials 2018, 11(4), 605 (2018); https://doi.org/10.3390/ma11040605 Jason V. Wandiyanto, Denver Linklater, Pallale G. Tharushi Perera, Anna Orlowska, Vi Khanh Truong, Helmut Thissen, Shahram Ghanaati, Vladimir A. Baulin, Russell J. Crawford, Saulius Juodkazis and Elena P. Ivanova Titanium is a biocompatible material that is frequently used for making implantable medical devices. Nanoengineering of the surface is the common method for increasing material biocompatibility, and while the nanostructured materials are well-known to represent attractive substrata for eukaryotic cells, very little information has been documented about the interaction between mammalian cells and bactericidal nanostructured surfaces. In this study, we investigated the effect of bactericidal titanium nanostructures on

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The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells

Elena Ivanova — Sun, 31 Dec 2017 13:53:54 +0000

Materials, 11(1) 60 (2018) Anna Orlowska, Pallale Tharushi Perera, Mohammad Al Kobaisi, Andre Dias, Huu Khuong Duy Nguyen, Shahram Ghanaati, Vladimir A. Baulin, Russell J. Crawford, Elena P. Ivanova Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings

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Bactericidal activity of self-assembled palmitic and stearic fatty acid crystals on highly ordered pyrolytic graphite

Elena Ivanova — Mon, 04 Sep 2017 14:01:32 +0000

Acta Biomaterialia, 59, 148-157 (2017). Elena P. Ivanova, Song Ha Nguyen, Yachong Guo, Vladimir A. Baulin, Hayden K. Webb, Vi Khanh Truong, Jason V. Wandiyanto, Christopher J. Garvey, Peter J. Mahon, David E. Mainwaring, Russell J. Crawford The wings of insects such as cicadas and dragonflies have been found to exhibit nanostructure arrays that are assembled from fatty acids, and that physically interact with and significantly disrupt bacterial cell membranes. Such mechanobactericidal surfaces are of significant interest, as they can kill bacteria without the need for antibacterial chemicals. Here, we report on the bactericidal effect of two main lipid components of

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The susceptibility of Staphylococcus aureus CIP 65.8 and Pseudomonas aeruginosa ATCC 9721 cells to the bactericidal action of nanostructured Calopteryx haemorrhoidalis damselfly wing surfaces

Elena Ivanova — Thu, 09 Mar 2017 17:42:07 +0000

Appl Microbiol Biotechnol (2017). doi:10.1007/s00253-017-8205-9 Vi Khanh Truong, Nipuni Mahanamanam Geeganagamage, Vladimir A. Baulin, Jitraporn Vongsvivut, Mark J. Tobin, Pere Luque, Russell J. Crawford, Elena P. Ivanova Nanostructured insect wing surfaces have been reported to possess the ability to resist bacterial colonization through the mechanical rupture of bacterial cells coming into contact with the surface. In this work, the susceptibility of physiologically young, mature and old Staphylococcus aureus CIP 65.8 and Pseudomonas aeruginosa ATCC 9721 bacterial cells, to the action of the bactericidal nano-pattern of damselfly Calopteryx haemorrhoidalis wing surfaces, was investigated. The results were obtained using several surface characterization

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Adsorption of Human Plasma Albumin and Fibronectin onto Nanostructured Black Silicon Surfaces

Elena Ivanova — Thu, 06 Oct 2016 19:35:12 +0000

Langmuir: 10.1021/acs.langmuir.6b02601 (2016) Duy H. K. Nguyen, Vy T. H. Pham, Mohammad Al Kobaisi, Chris M. Bhadra, Anna Orlowska, Shahram Ghanaati, Berardo Mario Manzi, Vladimir A Baulin, Saulius Juodkazis, Peter Kingshott, Russell J Crawford, and Elena P. Ivanova The protein adsorption of two human plasma proteins, albumin (Alb) and fibronectin (Fn) onto synthetic nanostructured bactericidal material, black silicon (bSi) surfaces (that contain an array of nanopillars) and silicon wafer (non-structured) surfaces, were investigated. The adsorption behaviour of Alb and Fn onto two types of substrata were studied using a combination of complementary analytical techniques. A two-step Alb adsorption mechanism onto the

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TV3 film in Terres de l’Ebre

Elena Ivanova — Tue, 08 Sep 2015 14:55:31 +0000

7 August 2015 – Massif Els Ports, Tarragona, Spain Outreach activity: Catalan TV channel TV3 film our expedition in Terres de l’Ebre to describe our research on dragonfly wings in Massif Els Ports. http://mp4-medium-dwn.media.tv3.cat/6/2/1440073012926.mp4 Highlight in newspapers and radio: TV3 TarragonaRadio URV Activa La Vanguardia Diari de Tarragona ElEconomista Comsciencia Ceics news Nacidigital Biblioteca Roquetes How it was filmed:

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Graphene Induces Formation of Pores That Kill Spherical and Rod-Shaped Bacteria

Elena Ivanova — Mon, 07 Sep 2015 11:35:57 +0000

ACS Nano, DOI: 10.1021/acsnano.5b03368 (2015) Vy T. H. Pham, Vi Khanh Truong, Matthew D. J. Quinn, Shannon M. Notley, Yachong Guo, Vladimir A. Baulin, Mohammad Al Kobaisi, Russell J. Crawford, and Elena P. Ivanova Pristine graphene, its derivatives, and composites have been widely reported to possess antibacterial properties. Most of the studies simulating the interaction between bacterial cell membranes and the surface of graphene have proposed that the graphene-induced bacterial cell death is caused either by (1) the insertion of blade-like graphene-based nanosheets or (2) the destructive extraction of lipid molecules by the presence of the lipophilic graphene. These simulation studies

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