Adsorption of Human Plasma Albumin and Fibronectin onto Nanostructured Black Silicon Surfaces

Langmuir: 10.1021/acs.langmuir.6b02601 (2016)

Duy H. K. Nguyen, Vy T. H. Pham, Mohammad Al Kobaisi, Chris M. Bhadra, Anna Orlowska, Shahram Ghanaati, Berardo Mario Manzi, Vladimir A Baulin, Saulius Juodkazis, Peter Kingshott, Russell J Crawford, and Elena P. Ivanova

The protein adsorption of two human plasma proteins, albumin (Alb) and fibronectin (Fn) onto synthetic nanostructured bactericidal material, black silicon (bSi) surfaces (that contain an array of nanopillars) and silicon wafer (non-structured) surfaces, were investigated. The adsorption behaviour of Alb and Fn onto two types of substrata were studied using a combination of complementary analytical techniques. A two-step Alb adsorption mechanism onto the bSi surface has been proposed. At low bulk concentrations (below 40 µg/mL), the Alb preferentially adsorbed at the base of the nanopillars. At higher bulk concentrations, the Alb adsorbed on the top of the nanopillars. In the case of Fn, the protein preferentially adsorbed on the top of the nanopillars, irrespective of its bulk concentration.

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DOI: 10.1021/acs.langmuir.6b02601


Elena Ivanova

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