Shahram Ghanaati

Frankfurt: Course on toxicology and molecular biology techniques

Shahram Ghanaati — Tue, 01 Sep 2015 19:44:35 +0000

3-5 December 2015 Core training course The course will cover such topics as methodology, models, and statistical estimation and hypothesis tests. Introductory course on toxicological evaluation of materials, basic cell and molecular biology techniques Given the large differences in background between experimental and theoretical/computational ESRs, training courses will aim to highlight these two areas at different levels. Thursday, 3th of December 2015 Frankfurt am Main, Germany Friday, 4th of December 2015 Frankfurt Goethe University – Hospital Saturday, 5th of December 2015 Frankfurt am Main, Germany Optional practical hands on course Hands on course will be available in the research labs

(Read more...)

Human cell culture models

Shahram Ghanaati — Sun, 08 Dec 2013 08:50:44 +0000

The REPAIR-lab will use human in vitro systems to study the cellular effects of the nano-objects developed by the other partner laboratories. The use of complete cells represents the most complex of the in vitro models available in the consortium. The REPAIR-lab has human cell culture models of different complexity, beginning with permanent cell lines of varying tissue origin, including endothelial, epithelial and connective tissue cells. These are useful models for the rapid testing of possible cytotoxic effects. The next level are human primary and early passage cells. Examples are various types of endothelial cells (EC), especially from the microcirculation

(Read more...)

ESR11-F: Delivery of molecular payloads through primary human cell membrane

Shahram Ghanaati — Thu, 05 Dec 2013 15:44:08 +0000

In-vitro experiments on living cells, toxicology studies Contact Andre Dias Objectives The goal is to compare and validate the synthetic lipid models to biologically relevant cell types and to investigate how different nano-objects interact with representative lipid single and bilayers compared to human cells with and without therapeutic incorporation. Tasks and methodology Cellular uptake of fluorescence-labelled nano-complexes will be checked by Delta Vision High Resolution microscopy and with an accompanied immunofluorescence counter-staining for intracellular organelles to identify distinct cellular endocytotic routes such as clathrin- or caveolae-dependent mechanisms or intracellular dispositions of the nanoparticles. Nanoparticles will be applied to the upper

(Read more...)

ESR12-F: Toxicity of nano-objects on human cells models

Shahram Ghanaati — Thu, 05 Dec 2013 15:40:48 +0000

In-vitro experiments on living cells, toxicology studies Contact Anna Orlowska Objectives Main objective is to compare, validate and corroborate the findings of the interaction of the nano-objects synthesised by the network to actual human cell models with the findings observed with the lipid membrane model systems. Tasks and methodology Recruited ESR will obtain experience in toxicity, cell- and molecular biological methodology and will be able to carry out toxicological evaluation of material, their attachment to, uptake or translocation across cells or barrier models. After nanomaterial exposure cell viability will be evaluated by standardized MTS-assay and the cellular membrane integrity of

(Read more...)