Frankfurt: Course on toxicology and molecular biology techniques


3-5 December 2015


Core training course

The course will cover such topics as methodology, models, and statistical estimation and hypothesis tests.

Introductory course on toxicological evaluation of materials, basic cell and molecular biology techniques

Given the large differences in background between experimental and theoretical/computational ESRs, training courses will aim to highlight these two areas at different levels.

Thursday, 3th of December 2015
Frankfurt am Main, Germany
18:00 Registration Open
19:00 Training Welcome and Opening Comments
Dr. Shahram Ghanaati
19:30 Dinner & visit to the Christmas market


Friday, 4th of December 2015
Frankfurt Goethe University – Hospital
09:00 Reception Open                            Building 23
09:30 Complementary Guided Tour of Medical Centre of Goethe University
Andre Dias
09:45 Coffee / Tea break
In vivo session
10:00 Introduction to clinical research
Dr. Shahram Ghanaati
10:45 Animal experimentation: Past and Future
Mike Barbeck
11:15 Discussion of the relevance of animal studies
11:45 In vivo concluding remarks
12:00 Lunch and exhibition of lab biomaterial collection
(Buffet lunch served)
In vitro session
13:30 The emergence of alternatives to animal research:
Introduction to in vitro technology
Anna Orlowska
14:00 Co-culture and three – dimensional culture models
Dr. Eva Dohle
15:00 Discussion of relevance of in vitro studies
15:30 Coffee/Tea Break
16:00 Molecular techniques in cancer tissue models
Dr. Patrick Booms
16:45 In vitro concluding remarks
17:00 Fascination motility
Prof. Dr. J. Bereiter-Hahn
18:00 Closing of theoretical part
Dr. Patrick Booms
20:00 Dinner (Restaurant near the hotel)
Dr. Shahram Ghanaati
Saturday, 5th of December 2015
Frankfurt am Main, Germany

Optional practical hands on course

Hands on course will be available in the research labs in the REPAIR lab in Frankfurt and will be given by senior academics.  This will include methods for the toxicological evaluation of materials, studies with primary human cells, their isolation, culture, determination and evaluation of the interaction consequences of cells with materials, gene expression studies with real-time PCR, confocal microscopy, SEM/TEM, ELISA etc. (all molecular and cell biological methodology and equipment is available).

The attendance to practical session is not mandatory and the activity is recommended for those who would like to gain insight into laboratory techniques available in FORM – Laboratory Frankfurt. The session is designed to give a brief overview of in vivo as well as in vitro techniques in the lab.

The Saturday session will start with the introduction to health and safety procedure. This activity is recommended to everyone who would like to participate in the following session.

Participants will be randomly split into smaller groups, with a maximum of 5 people per group. In this session, each attendee will get the opportunity to participate in three different sessions: PRF (Platelet Rich Fibrin), in vitro and in vivo techniques. Each part of the practical session will be held by a different person who will provide you with appropriate training.

Saturday, 5th of December 2015
Time Groups
1 2 3
09:00 Opening
09:15 Introduction to workplace health & safety
09:45 Coffee/Tea Break & Group division
10:00 PRF In vivo In vitro
12:00 Lunch
13:00 In vivo In vitro PRF
15:00 Coffee/Tea Break
15:30 In vitro PRF In vivo
17:30 Concluding remarks of practical session
We look forward to welcome you in our lab
We hope that this meeting will inspire discussion and provide a great opportunity for participants to get in touch in a nice, friendly and relaxing environment.


Shahram Ghanaati

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