Vladimir Baulin

Simulations of Protein Adsorption on Nanostructured Surfaces

Vladimir Baulin — Thu, 21 Mar 2019 08:57:14 +0000

Scientific Reports, 9, 4694 (2019) Berardo M. Manzi, Marco Werner, Elena P. Ivanova, Russell J. Crawford and Vladimir A. Baulin Recent technological advances have allowed the development of a new generation of nanostructured materials, such as those displaying both mechano-bactericidal activity and substrata that favor the growth of mammalian cells. Nanomaterials that come into contact with biological media such as blood first interact with proteins, hence understanding the process of adsorption of proteins onto these surfaces is highly important. The Random Sequential Adsorption (RSA) model for protein adsorption on flat surfaces was modified to account for nanostructured surfaces. Phenomena related

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The Interaction of Giant Unilamellar Vesicles (GUVs) with the Surface Nanostructures on Dragonfly Wings

Vladimir Baulin — Sat, 12 Jan 2019 11:12:52 +0000

Langmuir, January (2019) S. Cheeseman, Vi Khanh Truong, V. Walter, F. Thalmann, C. M. Marques, Eric Hanssen, J. Vongsvivut, M. Tobin, V. A. Baulin, S. Juodkazis, S. MacLaughlin, G. Bryant, R. J. Crawford, E. P. Ivanova The waxy epicuticle of dragonfly wings contains a unique nanostructured pattern that exhibits bactericidal properties. In light of emerging concerns of antibiotic resistance, these mechano-bactericidal surfaces represent a particularly novel solution by which bacterial colonization and the formation of biofilms on biomedical devices can be prevented. Pathogenic bacterial biofilms on medical implant surfaces cause a significant number of human deaths every year. The proposed

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High-Throughput 3D Visualization of Nanoparticles attached to the Surface of Red Blood Cells

Vladimir Baulin — Wed, 26 Dec 2018 14:21:44 +0000

Nanoscale, December (2018) André Dias, Marco Werner, Kevin Ward, Jean-Baptiste Fleury, Vladimir A. Baulin Blood circulation is the main distribution route for systemic delivery and the possibility to manipulate red blood cells by attaching nanoparticles to their surface provides a great opportunity for cargo delivery into tissues. Nanocarriers attached to red blood cells can be delivered to specific organs by orders of magnitude faster than if diluted in the blood. Another ad-vantage is a shielding from recognition of the immune system, thereby increasing the efficiency of delivery. We present a high-throughput microfluidic method that can monitor the shape of drifting

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Tension-Induced Translocation of Ultra-Short Carbon Nanotube through a Phospholipid Bilayer

Vladimir Baulin — Wed, 21 Nov 2018 06:21:48 +0000

ACS Nano, November (2018) Yachong Guo, Marco Werner, Ralf Seemann, Vladimir A Baulin, and Jean-Baptiste Fleury Increasing awareness of bioeffects and toxicity of nanomaterials interacting with cells puts in focus the mechanisms by which nanomaterials can cross lipid membranes. Apart from well-discussed energy-dependent endocytosis for large objects and passive diffusion through membranes by solute molecules, there can exist other translocation mechanisms based on physical principles. We show the importance of membrane tension on the translocation through lipid bilayers of ultra-short carbon nanotubes (USCNTs). By using a combination of a microfluidic setup and single chain mean field theory we observed that

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High Aspect Ratio Nanostructures Kill Bacteria via Storage and Release of Mechanical Energy

Vladimir Baulin — Mon, 25 Jun 2018 13:10:56 +0000

ACS Nano, 114(9), 2165–2173 (2018) Denver P. Linklater, Michael De Volder, Vladimir A Baulin, Marco Werner, Sarah Jessl, Mehdi Golozar, Laura Maggini, Sergey Rubanov, Eric Hanssen, Saulius Juodkazis, Elena P. Ivanova The properties of lipid bilayers in sucrose solutions have been intensely scrutinized over recent decades because of the importance of sugars in the field of biopreservation. However, a consensus has not yet been formed on the mechanisms of sugar-lipid interaction. Here, we present a study on the effect of sucrose on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers that combines calorimetry, spectral fluorimetry, and optical microscopy. Intriguingly, our results show a significant decrease in

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Structure and Chemical Organization in Damselfly Calopteryx haemorrhoidalis Wings: A Spatially Resolved FTIR and XRF Analysis with Synchrotron Radiation

Vladimir Baulin — Mon, 25 Jun 2018 13:03:38 +0000

Scientific Reports, 8, 8413 (2018) Susan Stuhr, Vi Khanh Truong, Jitraporn Vongsvivut, Tobias Senkbeil, Yang Yang, Mohammad Al Kobaisi, Vladimir A. Baulin, Marco Werner, Sergey Rubanov, Mark J. Tobin, Peter Cloetens, Axel Rosenhahn, Robert N. Lamb, Pere Luque, Richard Marchant, Elena P. Ivanova Insects represent the majority of known animal species and exploit a variety of fascinating nanotechnological concepts. We investigated the wings of the damselfly Calopteryx haemorrhoidalis, whose males have dark pigmented wings and females have slightly pigmented wings. We used scanning electron microscopy (SEM) and nanoscale synchrotron X-ray fluorescence (XRF) microscopy analysis for characterizing the nanostructure and the

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DPPC Bilayers in Solutions of High Sucrose Content

Vladimir Baulin — Mon, 25 Jun 2018 12:44:16 +0000

Biophysical J, 114(9), 2165–2173 (2018) Mattia I. Morandi, Mathieu Sommer, Monika Kluzek, Fabrice Thalmann, André P. Schroder, Carlos M. Marques The properties of lipid bilayers in sucrose solutions have been intensely scrutinized over recent decades because of the importance of sugars in the field of biopreservation. However, a consensus has not yet been formed on the mechanisms of sugar-lipid interaction. Here, we present a study on the effect of sucrose on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers that combines calorimetry, spectral fluorimetry, and optical microscopy. Intriguingly, our results show a significant decrease in the transition enthalpy but only a minor shift in the transition

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Mechano-bactericidal mechanism of graphene nanomaterials

Vladimir Baulin — Sat, 28 Apr 2018 12:48:05 +0000

Interface Focus, 8, 20170060, (2018) Denver P. Linklater, Vladimir A. Baulin, Saulius Juodkazis and Elena P. Ivanova Growing interest in the bactericidal effect of graphene and graphene-derived nanomaterials has led to the investigation and effective publication of the bactericidal effects of the substratum, many of which present highly conflicting material. The nature of bacterial cell death on graphene bio-interfaces, therefore, remains poorly understood. Here, we review recent findings on the bactericidal effect of graphene and graphene-derived nanomaterials, and proposed mechanisms of cell inactivation, due to mechanical contact with graphene materials, including lipid extraction, physical damage to membranes and pore formation. DOI: 10.1098/rsfs.2017.0060

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Study of melanin localization in the mature male Calopteryx haemorrhoidalis damselfly wings

Vladimir Baulin — Sat, 28 Apr 2018 12:38:09 +0000

J. Synchrotron Rad. 25, 874-877 (2018) Vi Khanh Truong, Jitraporn Vongsvivut, Nipuni Mahanamanam Geeganagamage, Mark J. Tobin, Pere Luque, Vladimir A. Baulin, Marco Werner, Shane Maclaughlin, Russell J. Crawford and Elena P. Ivanova Damselflies Calopteryx haemorrhoidalis exhibiting black wings are found in the western Mediterranean, Algeria, France, Italy, Spain and Monaco. Wing pigmentation is caused by the presence of melanin, which is involved in physiological processes including defence reactions, wound healing and sclerotization of the insect. Despite the important physiological roles of melanin, the presence and colour variation among males and females of the C. haemorrhoidalis species and the localization of the pigment within the wing membrane remain poorly

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Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces

Vladimir Baulin — Sat, 28 Apr 2018 12:27:39 +0000

Materials 2018, 11(4), 605 (2018); https://doi.org/10.3390/ma11040605 Jason V. Wandiyanto, Denver Linklater, Pallale G. Tharushi Perera, Anna Orlowska, Vi Khanh Truong, Helmut Thissen, Shahram Ghanaati, Vladimir A. Baulin, Russell J. Crawford, Saulius Juodkazis and Elena P. Ivanova Titanium is a biocompatible material that is frequently used for making implantable medical devices. Nanoengineering of the surface is the common method for increasing material biocompatibility, and while the nanostructured materials are well-known to represent attractive substrata for eukaryotic cells, very little information has been documented about the interaction between mammalian cells and bactericidal nanostructured surfaces. In this study, we investigated the effect of bactericidal titanium nanostructures on

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