Posts by Anna Orlowska published at ITN SNAL - Marie Curie Initial Training Network

Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces

Anna Orlowska — Sat, 28 Apr 2018 12:27:39 +0000

Materials 2018, 11(4), 605 (2018); https://doi.org/10.3390/ma11040605 Jason V. Wandiyanto, Denver Linklater, Pallale G. Tharushi Perera, Anna Orlowska, Vi Khanh Truong, Helmut Thissen, Shahram Ghanaati, Vladimir A. Baulin, Russell J. Crawford, Saulius Juodkazis and Elena P. Ivanova Titanium is a biocompatible material that is frequently used for making implantable medical devices. Nanoengineering of the surface is the common method for increasing material biocompatibility, and while the nanostructured materials are well-known to represent attractive substrata for eukaryotic cells, very little information has been documented about the interaction between mammalian cells and bactericidal nanostructured surfaces. In this study, we investigated the effect of bactericidal titanium nanostructures on

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The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells

Anna Orlowska — Sun, 31 Dec 2017 13:53:54 +0000

Materials, 11(1) 60 (2018) Anna Orlowska, Pallale Tharushi Perera, Mohammad Al Kobaisi, Andre Dias, Huu Khuong Duy Nguyen, Shahram Ghanaati, Vladimir A. Baulin, Russell J. Crawford, Elena P. Ivanova Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings

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Sugar-based collagen membrane cross-linking increases barrier capacity of membranes

Anna Orlowska — Thu, 14 Dec 2017 16:15:51 +0000

Clin. Oral Invest., (2017) Po-ju Chia-LaiAnna OrlowskaSarah Al-MaawiAndre DiasYunxin ZhangXuejiu WangNiklas ZenderRobert SaderC. James KirkpatrickShahram Ghanaati This study examines the permeability and barrier capacity of a sugar cross-linked resorbable collagen membrane ex vivo and in vivo. In an ex vivo study, injectable platelet-rich fibrin (i-PRF), a peripheral blood-derived human leukocyte-and-platelet-rich plasma was used to analyze membrane permeability. in vivo subcutaneous implantation in Wistar rats (n = 4 per time point and group) was used to investigate the barrier capacity of the membrane. The induced in vivo cellular reaction was evaluated at 3, 15, and 30 days and compared to sham OP (control)

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Reduction of the relative centrifugal force influences cell number and growth factor release within injectable PRF-based matrices

Anna Orlowska — Thu, 02 Nov 2017 16:10:22 +0000

J Mater Sci: Mater Med, 28, 188 (2017) Simon Wend, Alica Kubesch, Anna Orlowska, Sarah Al-Maawi, Niklas Zender, Andre Dias, Richard J. Miron, Robert Sader, Patrick Booms, C. James Kirkpatrick, Joseph Choukroun, Shahram Ghanaati Platelet rich fibrin (PRF) is a blood concentrate system obtained by centrifugation of peripheral blood. First PRF matrices exhibited solid fibrin scaffold, more recently liquid PRF-based matrix was developed by reducing the relative centrifugation force and time. The aim of this study was to systematically evaluate the influence of RCF (relative centrifugal force) on cell types and growth factor release within injectable PRF- in the range of

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In vivo cellular reactions to different biomaterials—Physiological and pathological aspects and their consequences

Anna Orlowska — Fri, 14 Jul 2017 11:11:05 +0000

Seminars in Immunology, DOI: 10.1016/j.smim.2017.06.001 (2017). Sarah Al-Maawi, Anna Orlowska, Robert Sader, C. James Kirkpatrick, Shahram Ghanaati Biomaterials are widely used in guided bone regeneration (GBR) and guided tissue regeneration (GTR). After application, there is an interaction between the host immune system and the implanted biomaterial, leading to a biomaterial-specific cellular reaction. The present review focuses on cellular reactions to numerous biomaterials in vivo with consideration of different implantation models and microenvironments in different species, such as subcutaneous implantation in mice and rats, a muscle model in goats and a femur model in rabbits. Additionally, cellular reactions to different biomaterials

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Frankfurt: Course on toxicology and molecular biology techniques

Anna Orlowska — Tue, 01 Sep 2015 19:44:35 +0000

3-5 December 2015 Core training course The course will cover such topics as methodology, models, and statistical estimation and hypothesis tests. Introductory course on toxicological evaluation of materials, basic cell and molecular biology techniques Given the large differences in background between experimental and theoretical/computational ESRs, training courses will aim to highlight these two areas at different levels. Thursday, 3th of December 2015 Frankfurt am Main, Germany Friday, 4th of December 2015 Frankfurt Goethe University – Hospital Saturday, 5th of December 2015 Frankfurt am Main, Germany Optional practical hands on course Hands on course will be available in the research labs

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ESR12-F: Toxicity of nano-objects on human cells models

Anna Orlowska — Thu, 05 Dec 2013 15:40:48 +0000

In-vitro experiments on living cells, toxicology studies Contact Anna Orlowska Objectives Main objective is to compare, validate and corroborate the findings of the interaction of the nano-objects synthesised by the network to actual human cell models with the findings observed with the lipid membrane model systems. Tasks and methodology Recruited ESR will obtain experience in toxicity, cell- and molecular biological methodology and will be able to carry out toxicological evaluation of material, their attachment to, uptake or translocation across cells or barrier models. After nanomaterial exposure cell viability will be evaluated by standardized MTS-assay and the cellular membrane integrity of

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