Kinetic evolution of DOPC lipid bilayers exposed to α-cyclodextrins

Soft Matter, , (2018)

Monika Kluzek, Marc Schmutz, Carlos M. Marques and Fabrice Thalmann

Cyclodextrins are cyclic oligosaccharides capable of forming inclusion complexes with a variety of molecules, and as such have been recognized as a pharmaceutical and biotechnological asset. Cyclodextrins are known to interact with the components of cell membranes, and this correlates with a significant degree of cytotoxicity. In this work, we report on the mechanism of degradation of a model dioleoyl-phosphatidylcholine (DOPC) bilayer exposed to a solution with increasing con- centrations of α-cyclodextrins. By combining optical fluorescence microscopy and quartz-crystal microbalance experiments, we study the evolution of supported lipid bilayers (SLBs) and giant unilamellar vesicles (GUVs). The rate of lipid removal is found to display a strong nonlinear de- pendence in the cyclodextrin concentration. A mechanism involving lipid aggregates is proposed.

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DOI: 10.1039/C8SM01013G

Monika Kluzek

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