Experimental methods and characterisation techniques
We have previously demonstrated that certain combinations of lipids within a liposome formulation enable the highly effective delivery of RNA liposome vaccines to cells. However, such formulations have to date only been prepared at laboratory scale and methods are required whereby they can be manufactured in sufficient quantities for clinical trials and eventual marketing. Such methods must enable the formulation of RNA in high yield, due to the high cost of both the active ingredient and the lipids involved in the formulation, and result in a thermally stable formulation in which liposomes neither agglomerate nor degrade. The efforts in this project will be focused on identifying improved liposome manufacturing methods.