Delivery of molecular payloads through primary human cell membrane
- Interests: Molecular biology, medicine, veterinary
- Supervisors: Shahram Ghanaati, Elena Ivanova
- Host institution: Universitätsklinikum Frankfurt
Short Bio
Anna Orlowska background combines veterinary (focused on toxicology and including practical experience with laboratory animals), pathology and medicine with a strong emphasis on interdisciplinary research work – with special interest in cancer research, due to recent experience with in vitro experiments using living (cancer) cells and subsequent functional assays in the FORM laboratory at the Medical Centre of University Goethe University Frankfurt.
During the veterinary degree Anna attended the ERASMUS Program in Afyon Kocatepe University, Turkey, where she was heavily involved in all aspects of the laboratory work as well as in performing surgeries on animals. Following this experience, she decided to pursue her interest in research and managed to secure a position at the Royal Veterinary College in London, where she earned a further intercalated BSc. degree in Veterinary Pathology. This period of study provided an exceptional opportunity to work with top researchers in their field. The programme was project-centered and enabled her to work independently on full-time research in one of the UK government’s Level 3 Biosafety facilities. For the last four months Anna has been working within the Facial Oral Regenerative Medicine (FORM) in Frankfurt, which she joined due to her increasing focus on tissue regeneration, cell cultures and biomaterials. Her experience in FORM has been extremely engaging and has provided another excellent learning opportunity working with talented and dedicated professionals in the laboratory.
2014-present | Marie Skłodowska-Curie Actions Early Stage Researcher. Medical Center, Universitaet Frankfurt, Germany |
2011-2012 | B.Sc. degree. Royal Veterinary College, University of London, UK Intercalated BSc, Veterinary Pathology, London, UK |
2009-2011 | DVM (Doctor of Veterinary Medicine). Veterinary Medicine (degree certificate received in 2013), Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. |
2010-2011 | Erasmus Student. Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey |
2004-2009 | Student. Warsaw University of Life Science, Warsaw, Poland. |
SNAL Project
- ESR12-F: Toxicity of nano-objects on human cells models
In-vitro experiments on living cells, toxicology studies Contact Anna Orlowska Objectives Main objective is to compare, validate and corroborate the findings of the interaction of the nano-objects synthesised by the network to actual human cell models with the findings observed with the lipid membrane model systems. Tasks and methodology Recruited ESR will obtain experience in toxicity, cell- and molecular biological methodology ...
Read more
Publications
- Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces
Materials 2018, 11(4), 605 (2018); https://doi.org/10.3390/ma11040605 Jason V. Wandiyanto, Denver Linklater, Pallale G. Tharushi Perera, Anna Orlowska, Vi Khanh Truong, Helmut Thissen, Shahram Ghanaati, Vladimir ...
- The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells
Materials, 11(1) 60 (2018) Anna Orlowska, Pallale Tharushi Perera, Mohammad Al Kobaisi, Andre Dias, Huu Khuong Duy Nguyen, Shahram Ghanaati, Vladimir A. Baulin, ...
- Sugar-based collagen membrane cross-linking increases barrier capacity of membranes
Clin. Oral Invest., (2017) Po-ju Chia-LaiAnna OrlowskaSarah Al-MaawiAndre DiasYunxin ZhangXuejiu WangNiklas ZenderRobert SaderC. James KirkpatrickShahram Ghanaati This study examines the permeability and ...
- Reduction of the relative centrifugal force influences cell number and growth factor release within injectable PRF-based matrices
J Mater Sci: Mater Med, 28, 188 (2017) Simon Wend, Alica Kubesch, Anna Orlowska, Sarah Al-Maawi, Niklas Zender, Andre Dias, Richard J. ...
- In vivo cellular reactions to different biomaterials—Physiological and pathological aspects and their consequences
Seminars in Immunology, DOI: 10.1016/j.smim.2017.06.001 (2017). Sarah Al-Maawi, Anna Orlowska, Robert Sader, C. James Kirkpatrick, Shahram Ghanaati Biomaterials are widely used in ...
- Adsorption of Human Plasma Albumin and Fibronectin onto Nanostructured Black Silicon Surfaces
Langmuir: 10.1021/acs.langmuir.6b02601 (2016) Duy H. K. Nguyen, Vy T. H. Pham, Mohammad Al Kobaisi, Chris M. Bhadra, Anna Orlowska, Shahram Ghanaati, Berardo ...
- “Race for the surface”: eukaryotic cells can win
ACS Appl. Mater. Interfaces: 8(34) 22025-22031 (2016) Vy T. H. Pham, Vi Khanh Truong, Anna Orlowska, Shahram Ghanaati, Mike Barbeck, Patrick ...